﻿                         序列表

<110>  凯莱英医药集团（天津）股份有限公司
       凯莱英生命科学技术（天津）有限公司
       天津凯莱英制药有限公司
       凯莱英医药化学（阜新）技术有限公司
       吉林凯莱英医药化学有限公司
 
<120>  双羰基还原酶突变体及其应用

<130>  LZ140958CN07

<160>  56    

<170>  PatentIn version 3.3

<210>  1
<211>  286
<212>  PRT
<213>  人工序列

<220>
<223>  双羰基还原酶突变体

<400>  1

Met Thr Glu Leu Lys Gln Ile Thr Val Leu Gly Thr Gly Val Leu Gly 
1               5                   10                  15      


Ser Gln Ile Ala Tyr Gln Thr Ala Cys His Gly Phe Asp Val Val Ala 
            20                  25                  30          


Tyr Asp Ile Asn Ala Glu Val Ile Glu Lys Ala Lys Ala Arg Phe Asp 
        35                  40                  45              


Ser Leu Ala Ala Ala Tyr Lys Ala Glu Asn Val Glu Gly Ala Lys Glu 
    50                  55                  60                  


Gly Lys Ala Asp Glu Ala Leu Gln Arg Ile Thr Tyr Ser Tyr Asp Leu 
65                  70                  75                  80  


Gly Glu Ala Val Ala Lys Ala Asp Leu Val Ile Glu Ala Ile Pro Glu 
                85                  90                  95      


Asp Ile Ala Ile Lys Arg Asp Thr Tyr Glu Lys Leu Ala Thr Val Ala 
            100                 105                 110         


Pro Glu His Thr Val Phe Ala Thr Asn Ser Ser Thr Leu Leu Pro Ser 
        115                 120                 125             


Asp Leu Lys Glu Phe Thr Gly Arg Pro Glu Lys Phe Leu Ala Leu His 
    130                 135                 140                 


Phe Ala Asn His Val Trp Val Asn Asn Thr Ala Glu Val Met Gly Thr 
145                 150                 155                 160 


Glu Ser Thr Asp Pro Ala Val Tyr Arg Glu Val Val Glu Phe Ala Lys 
                165                 170                 175     


Asn Ile Gly Met Val Pro Ile Glu Leu Lys Lys Glu Lys Ala Gly Tyr 
            180                 185                 190         


Val Leu Asn Ser Leu Leu Val Pro Leu Leu Asn Ala Ala Ser Asp Leu 
        195                 200                 205             


Leu Ile Asp Gly Ile Ala Asp Pro Asp Met Val Asp Lys Thr Trp Arg 
    210                 215                 220                 


Ile Gly Thr Gly Ala Pro Trp Gly Pro Phe Gln Ile Met Asp Val Val 
225                 230                 235                 240 


Gly Leu Thr Thr Val Tyr Asn Ile Ser Ser Gln Gly Gly Glu Lys Gln 
                245                 250                 255     


Arg Glu Phe Ala Asp Tyr Ile Lys Lys Asn Tyr Ile Asp Glu Gly Lys 
            260                 265                 270         


Leu Gly Val Ala Val Gly Asp Gly Phe Tyr Asn Tyr Lys Gly 
        275                 280                 285     


<210>  2
<211>  286
<212>  PRT
<213>  人工序列

<220>
<223>  双羰基还原酶突变体

<400>  2

Met Thr Glu Leu Lys Gln Ile Thr Val Leu Gly Thr Gly Val Leu Gly 
1               5                   10                  15      


Ser Gln Ile Ala Tyr Gln Thr Ala Cys His Gly Phe Asp Val Val Ala 
            20                  25                  30          


Tyr Asp Ile Asn Ala Glu Val Ile Glu Lys Ala Lys Ala Arg Phe Asp 
        35                  40                  45              


Ser Leu Ala Ala Ala Tyr Lys Ala Glu Asn Val Glu Gly Ala Lys Glu 
    50                  55                  60                  


Gly Lys Ala Asp Glu Ala Leu Gln Arg Ile Thr Tyr Ser Tyr Asp Leu 
65                  70                  75                  80  


Gly Glu Ala Val Ala Lys Ala Asp Leu Val Ile Glu Ala Val Pro Glu 
                85                  90                  95      


Asp Ile Ala Ile Lys Arg Asp Thr Tyr Glu Lys Leu Ala Thr Val Ala 
            100                 105                 110         


Pro Glu His Thr Val Phe Ala Thr Asn Ser Ser Thr Leu Leu Pro Ser 
        115                 120                 125             


Asp Leu Lys Glu Phe Thr Gly Arg Pro Glu Lys Phe Leu Ala Leu His 
    130                 135                 140                 


Phe Ala Asn His Val Trp Val Asn Asn Thr Ala Glu Val Met Gly Thr 
145                 150                 155                 160 


Glu Ser Thr Asp Pro Ala Val Tyr Arg Glu Val Val Glu Phe Ala Lys 
                165                 170                 175     


Asn Ile Gly Met Val Pro Ile Glu Leu Lys Lys Glu Lys Ala Gly Tyr 
            180                 185                 190         


Val Leu Asn Ser Leu Leu Val Pro Leu Leu Asn Ala Ala Ser Asp Leu 
        195                 200                 205             


Leu Ile Asp Gly Ile Ala Asp Pro Asp Met Val Asp Lys Thr Trp Arg 
    210                 215                 220                 


Ile Gly Thr Gly Ala Pro Trp Gly Pro Phe Gln Ile Met Asp Val Val 
225                 230                 235                 240 


Gly Leu Thr Thr Val Tyr Asn Ile Ser Ser Gln Gly Gly Glu Lys Gln 
                245                 250                 255     


Arg Glu Phe Ala Asp Tyr Ile Lys Lys Asn Tyr Ile Asp Glu Gly Lys 
            260                 265                 270         


Leu Gly Val Ala Val Gly Asp Gly Phe Tyr Asn Tyr Lys Gly 
        275                 280                 285     


<210>  3
<211>  286
<212>  PRT
<213>  人工序列

<220>
<223>  双羰基还原酶突变体

<400>  3

Met Thr Glu Leu Lys Gln Ile Thr Val Leu Gly Thr Gly Val Leu Gly 
1               5                   10                  15      


Ser Gln Ile Ala Tyr Gln Thr Ala Cys His Gly Phe Asp Val Val Ala 
            20                  25                  30          


Tyr Asp Ile Asn Ala Glu Val Ile Glu Lys Ala Lys Ala Arg Phe Asp 
        35                  40                  45              


Ser Leu Ala Ala Ala Tyr Lys Ala Glu Asn Val Glu Gly Ala Lys Glu 
    50                  55                  60                  


Gly Lys Ala Asp Glu Ala Leu Gln Arg Ile Thr Tyr Ser Tyr Asp Leu 
65                  70                  75                  80  


Gly Glu Ala Val Ala Lys Ala Asp Leu Val Ile Glu Ala Val Pro Glu 
                85                  90                  95      


Asp Ile Ala Ile Lys Arg Asp Thr Tyr Glu Lys Leu Ala Thr Val Ala 
            100                 105                 110         


Pro Glu His Thr Val Phe Ala Thr Asn Ser Ser Thr Leu Leu Pro Ser 
        115                 120                 125             


Asp Leu Lys Glu Phe Thr Gly Arg Pro Glu Lys Phe Leu Ala Leu His 
    130                 135                 140                 


Phe Ala Asn His Val Trp Gln Asn Asn Thr Ala Glu Val Met Gly Thr 
145                 150                 155                 160 


Glu Ser Thr Asp Pro Ala Val Tyr Arg Glu Val Val Glu Phe Ala Lys 
                165                 170                 175     


Asn Ile Gly Met Val Pro Ile Glu Leu Lys Lys Glu Lys Ala Gly Tyr 
            180                 185                 190         


Val Leu Asn Ser Leu Leu Val Pro Leu Leu Asn Ala Ala Ser Asp Leu 
        195                 200                 205             


Leu Ile Asp Gly Ile Ala Asp Pro Asp Met Val Asp Lys Thr Trp Arg 
    210                 215                 220                 


Ile Gly Thr Gly Ala Pro Trp Gly Pro Phe Gln Ile Met Asp Val Val 
225                 230                 235                 240 


Gly Leu Thr Thr Val Tyr Asn Ile Ser Ser Gln Gly Gly Glu Lys Gln 
                245                 250                 255     


Arg Glu Phe Ala Asp Tyr Ile Lys Lys Asn Tyr Ile Asp Glu Gly Lys 
            260                 265                 270         


Leu Gly Val Ala Val Gly Asp Gly Phe Tyr Asn Tyr Lys Gly 
        275                 280                 285     


<210>  4
<211>  286
<212>  PRT
<213>  人工序列

<220>
<223>  双羰基还原酶突变体

<400>  4

Met Thr Glu Leu Lys Gln Ile Thr Val Leu Gly Thr Gly Val Leu Gly 
1               5                   10                  15      


Ser Gln Ile Ala Tyr Gln Thr Ala Cys His Gly Phe Asp Val Val Ala 
            20                  25                  30          


Tyr Asp Ile Asn Ala Glu Val Ile Glu Lys Ala Lys Ala Arg Phe Asp 
        35                  40                  45              


Ser Leu Ala Ala Ala Tyr Lys Ala Glu Asn Val Glu Gly Ala Lys Glu 
    50                  55                  60                  


Gly Lys Ala Asp Glu Ala Leu Gln Arg Ile Thr Tyr Ser Tyr Asp Leu 
65                  70                  75                  80  


Gly Glu Ala Val Ala Lys Ala Asp Leu Val Ile Glu Ala Ile Pro Glu 
                85                  90                  95      


Asp Ile Ala Ile Lys Arg Asp Thr Tyr Glu Lys Leu Ala Thr Val Ala 
            100                 105                 110         


Pro Glu His Thr Val Phe Ala Thr Asn Ser Ser Thr Leu Leu Pro Ser 
        115                 120                 125             


Asp Leu Lys Glu Phe Thr Gly Arg Pro Glu Lys Phe Leu Ala Leu His 
    130                 135                 140                 


Phe Ala Asn His Val Trp Val Asn Asn Thr Ala Glu Val Met Gly Thr 
145                 150                 155                 160 


Glu Ser Thr Asp Pro Ala Val Tyr Arg Glu Val Val Glu Phe Ala Lys 
                165                 170                 175     


Asn Ile Gly Met Val Pro Ile Glu Leu Lys Lys Glu Lys Ala Gly Tyr 
            180                 185                 190         


Val Leu Asn Ser Leu Leu Val Pro Leu Leu Asn Ala Ala Ser Asp Leu 
        195                 200                 205             


Leu Ile Asp Gly Ile Ala Asp Pro Asp Met Val Asp Lys Thr Trp Arg 
    210                 215                 220                 


Ile Gly Thr Gly Ala Pro Phe Gly Pro Phe Gln Ile Met Asp Ile Val 
225                 230                 235                 240 


Gly Leu Thr Thr Val Tyr Asn Ile Ser Ser Gln Gly Gly Glu Lys Gln 
                245                 250                 255     


Lys Glu Phe Ala Asp Tyr Ile Lys Lys Asn Tyr Ile Asp Glu Gly Lys 
            260                 265                 270         


Leu Gly Val Ala Val Gly Asp Gly Phe Tyr Asn Tyr Lys Gly 
        275                 280                 285     


<210>  5
<211>  286
<212>  PRT
<213>  人工序列

<220>
<223>  双羰基还原酶突变体

<400>  5

Met Thr Glu Leu Lys Gln Ile Thr Val Leu Gly Thr Gly Val Leu Gly 
1               5                   10                  15      


Ser Gln Ile Ala Tyr Gln Thr Ala Cys His Gly Phe Asp Val Val Ala 
            20                  25                  30          


Tyr Asp Ile Asn Ala Glu Val Ile Glu Lys Ala Lys Ala Arg Phe Asp 
        35                  40                  45              


Ser Leu Ala Ala Ala Tyr Lys Ala Glu Asn Val Glu Gly Ala Lys Glu 
    50                  55                  60                  


Gly Lys Ala Asp Glu Ala Leu Gln Arg Ile Thr Tyr Ser Tyr Asp Leu 
65                  70                  75                  80  


Gly Glu Ala Val Ala Lys Ala Asp Leu Val Ile Glu Ala Ile Pro Glu 
                85                  90                  95      


Asp Ile Ala Ile Lys Arg Asp Thr Tyr Glu Lys Leu Ala Thr Val Ala 
            100                 105                 110         


Pro Glu His Thr Val Phe Ala Thr Asn Ser Ser Thr Leu Leu Pro Ser 
        115                 120                 125             


Asp Leu Lys Glu Phe Thr Gly Arg Pro Glu Lys Phe Leu Ala Leu His 
    130                 135                 140                 


Phe Ala Asn His Val Trp Gln Asn Asn Thr Ala Glu Val Met Gly Thr 
145                 150                 155                 160 


Glu Ser Thr Asp Pro Ala Val Tyr Arg Glu Val Val Glu Phe Ala Lys 
                165                 170                 175     


Asn Ile Gly Met Val Pro Ile Glu Leu Lys Lys Glu Lys Ala Gly Tyr 
            180                 185                 190         


Val Leu Asn Ser Leu Leu Val Pro Leu Leu Asn Ala Ala Ser Asp Leu 
        195                 200                 205             


Leu Ile Asp Gly Ile Ala Asp Pro Asp Met Val Asp Lys Thr Trp Arg 
    210                 215                 220                 


Ile Gly Thr Gly Ala Pro Phe Gly Pro Phe Gln Ile Met Asp Val Val 
225                 230                 235                 240 


Gly Leu Thr Thr Val Tyr Asn Ile Ser Ser Gln Gly Gly Glu Lys Gln 
                245                 250                 255     


Lys Glu Phe Ala Asp Tyr Ile Lys Lys Asn Tyr Ile Asp Glu Gly Lys 
            260                 265                 270         


Leu Gly Val Ala Val Gly Asp Gly Phe Tyr Asn Tyr Lys Gly 
        275                 280                 285     


<210>  6
<211>  286
<212>  PRT
<213>  人工序列

<220>
<223>  双羰基还原酶突变体

<400>  6

Met Thr Glu Leu Lys Gln Ile Thr Val Leu Gly Thr Gly Val Leu Gly 
1               5                   10                  15      


Ser Gln Ile Ala Tyr Gln Thr Ala Cys His Gly Phe Asp Val Val Ala 
            20                  25                  30          


Tyr Asp Ile Asn Ala Glu Val Ile Glu Lys Ala Lys Ala Arg Phe Asp 
        35                  40                  45              


Ser Leu Ala Ala Ala Tyr Lys Ala Glu Asn Val Glu Gly Ala Lys Glu 
    50                  55                  60                  


Gly Lys Ala Asp Glu Ala Leu Gln Arg Ile Thr Tyr Ser Tyr Asp Leu 
65                  70                  75                  80  


Gly Glu Ala Val Ala Lys Ala Asp Leu Val Ile Glu Ala Val Pro Glu 
                85                  90                  95      


Asp Ile Ala Ile Lys Arg Asp Thr Tyr Glu Lys Leu Ala Thr Val Ala 
            100                 105                 110         


Pro Glu His Thr Val Phe Ala Thr Asn Ser Ser Thr Leu Leu Pro Ser 
        115                 120                 125             


Asp Leu Lys Glu Phe Thr Gly Arg Pro Glu Lys Phe Leu Ala Leu His 
    130                 135                 140                 


Phe Ala Asn His Val Trp Gln Asn Asn Thr Ala Glu Val Met Gly Thr 
145                 150                 155                 160 


Glu Ser Thr Asp Pro Ala Val Tyr Arg Glu Val Val Glu Phe Ala Lys 
                165                 170                 175     


Asn Ile Gly Met Val Pro Ile Glu Leu Lys Lys Glu Lys Ala Gly Tyr 
            180                 185                 190         


Val Leu Asn Ser Leu Leu Val Pro Leu Leu Asn Ala Ala Ser Asp Leu 
        195                 200                 205             


Leu Ile Asp Gly Ile Ala Asp Pro Asp Met Val Asp Lys Thr Trp Arg 
    210                 215                 220                 


Ile Gly Thr Gly Ala Pro Phe Gly Pro Phe Gln Ile Met Asp Val Val 
225                 230                 235                 240 


Gly Leu Thr Thr Val Tyr Asn Ile Ser Ser Gln Gly Gly Glu Lys Gln 
                245                 250                 255     


Arg Glu Phe Ala Asp Tyr Ile Lys Lys Asn Tyr Ile Asp Glu Gly Lys 
            260                 265                 270         


Leu Gly Val Ala Val Gly Asp Gly Phe Tyr Asn Tyr Lys Gly 
        275                 280                 285     


<210>  7
<211>  286
<212>  PRT
<213>  红串红球菌

<400>  7

Met Thr Glu Leu Lys Gln Ile Thr Val Leu Gly Thr Gly Val Leu Gly 
1               5                   10                  15      


Ser Gln Ile Ala Tyr Gln Thr Ala Cys His Gly Phe Asp Val Val Ala 
            20                  25                  30          


Tyr Asp Ile Asn Ala Glu Val Ile Glu Lys Ala Lys Ala Arg Phe Asp 
        35                  40                  45              


Ser Leu Ala Ala Ala Tyr Lys Ala Glu Asn Val Glu Gly Ala Lys Glu 
    50                  55                  60                  


Gly Lys Ala Asp Glu Ala Leu Gln Arg Ile Thr Tyr Ser Tyr Asp Leu 
65                  70                  75                  80  


Gly Glu Ala Val Ala Lys Ala Asp Leu Val Ile Glu Ala Ile Pro Glu 
                85                  90                  95      


Asp Ile Ala Ile Lys Arg Asp Thr Tyr Glu Lys Leu Ala Thr Val Ala 
            100                 105                 110         


Pro Glu His Thr Val Phe Ala Thr Asn Ser Ser Thr Leu Leu Pro Ser 
        115                 120                 125             


Asp Leu Lys Glu Phe Thr Gly Arg Pro Glu Lys Phe Leu Ala Leu His 
    130                 135                 140                 


Phe Ala Asn His Val Trp Val Asn Asn Thr Ala Glu Val Met Gly Thr 
145                 150                 155                 160 


Glu Ser Thr Asp Pro Ala Val Tyr Arg Glu Val Val Glu Phe Ala Lys 
                165                 170                 175     


Asn Ile Gly Met Val Pro Ile Glu Leu Lys Lys Glu Lys Ala Gly Tyr 
            180                 185                 190         


Val Leu Asn Ser Leu Leu Val Pro Leu Leu Asn Ala Ala Ser Asp Leu 
        195                 200                 205             


Leu Ile Asp Gly Ile Ala Asp Pro Asp Met Val Asp Lys Thr Trp Arg 
    210                 215                 220                 


Ile Gly Thr Gly Ala Pro Phe Gly Pro Phe Gln Ile Met Asp Val Val 
225                 230                 235                 240 


Gly Leu Thr Thr Val Tyr Asn Ile Ser Ser Gln Gly Gly Glu Lys Gln 
                245                 250                 255     


Arg Glu Phe Ala Asp Tyr Ile Lys Lys Asn Tyr Ile Asp Glu Gly Lys 
            260                 265                 270         


Leu Gly Val Ala Val Gly Asp Gly Phe Tyr Asn Tyr Lys Gly 
        275                 280                 285     


<210>  8
<211>  861
<212>  DNA
<213>  红串红球菌

<400>  8
atgactgaac tgaagcagat caccgttctg ggtaccggag ttctcggctc acagatcgcc     60

tatcagaccg cctgtcacgg tttcgacgtc gtcgcgtacg acatcaacgc cgaggtcatc    120

gaaaaggcca aggctcggtt cgactcgttg gccgcggcct acaaggccga gaacgtcgag    180

ggtgccaagg aaggcaaggc tgacgaagcg ctgcaacgta ttacgtactc gtacgatcta    240

ggcgaagccg tcgccaaggc cgacctggtc atcgaggcaa ttcccgagga catcgccatc    300

aagcgcgaca cctacgagaa gcttgccacg gttgctcctg agcacacggt gttcgctacc    360

aactcctcga cgctgctgcc gagcgatctc aaggagttca ccggccgtcc cgagaagttc    420

ctcgcactgc acttcgcaaa tcacgtgtgg gtcaacaaca ctgccgaggt catgggcacc    480

gagtccaccg accccgccgt gtaccgcgag gtcgtcgagt tcgcgaagaa catcggcatg    540

gtgccgatcg aactcaagaa ggagaaggcg ggctacgtac tcaactcgct cctggtcccg    600

ctcctcaacg cggcctccga cctgctgatc gacggcatcg ccgatcccga catggtcgac    660

aagacgtggc gtatcggcac cggagccccg ttcggcccct tccagatcat ggacgtcgtc    720

gggttgacca ccgtctacaa catctcctcc cagggcggcg agaagcagcg cgaattcgcc    780

gactacatca agaagaacta catcgacgag ggcaagctcg gcgttgctgt cggcgacggc    840

ttctacaact acaagggctg a                                              861


<210>  9
<211>  861
<212>  DNA
<213>  人工序列

<220>
<223>  双羰基还原酶突变体编码序列

<400>  9
atgactgaac tgaagcagat caccgttctg ggtaccggag ttctcggctc acagatcgcc     60

tatcagaccg cctgtcacgg tttcgacgtc gtcgcgtacg acatcaacgc cgaggtcatc    120

gaaaaggcca aggctcggtt cgactcgttg gccgcggcct acaaggccga gaacgtcgag    180

ggtgccaagg aaggcaaggc tgacgaagcg ctgcaacgta ttacgtactc gtacgatcta    240

ggcgaagccg tcgccaaggc cgacctggtc atcgaggcaa ttcccgagga catcgccatc    300

aagcgcgaca cctacgagaa gcttgccacg gttgctcctg agcacacggt gttcgctacc    360

aactcctcga cgctgctgcc gagcgatctc aaggagttca ccggccgtcc cgagaagttc    420

ctcgcactgc acttcgcaaa tcacgtgtgg gtcaacaaca ctgccgaggt catgggcacc    480

gagtccaccg accccgccgt gtaccgcgag gtcgtcgagt tcgcgaagaa catcggcatg    540

gtgccgatcg aactcaagaa ggagaaggcg ggctacgtac tcaactcgct cctggtcccg    600

ctcctcaacg cggcctccga cctgctgatc gacggcatcg ccgatcccga catggtcgac    660

aagacgtggc gtatcggcac cggagccccg tggggcccct tccagatcat ggacgtcgtc    720

gggttgacca ccgtctacaa catctcctcc cagggcggcg agaagcagcg cgaattcgcc    780

gactacatca agaagaacta catcgacgag ggcaagctcg gcgttgctgt cggcgacggc    840

ttctacaact acaagggctg a                                              861


<210>  10
<211>  861
<212>  DNA
<213>  人工序列

<220>
<223>  双羰基还原酶突变体编码序列


<220>
<221>  misc_feature
<222>  (282)..(282)
<223>  n=a、c、g或t

<400>  10
atgactgaac tgaagcagat caccgttctg ggtaccggag ttctcggctc acagatcgcc     60

tatcagaccg cctgtcacgg tttcgacgtc gtcgcgtacg acatcaacgc cgaggtcatc    120

gaaaaggcca aggctcggtt cgactcgttg gccgcggcct acaaggccga gaacgtcgag    180

ggtgccaagg aaggcaaggc tgacgaagcg ctgcaacgta ttacgtactc gtacgatcta    240

ggcgaagccg tcgccaaggc cgacctggtc atcgaggcag tncccgagga catcgccatc    300

aagcgcgaca cctacgagaa gcttgccacg gttgctcctg agcacacggt gttcgctacc    360

aactcctcga cgctgctgcc gagcgatctc aaggagttca ccggccgtcc cgagaagttc    420

ctcgcactgc acttcgcaaa tcacgtgtgg gtcaacaaca ctgccgaggt catgggcacc    480

gagtccaccg accccgccgt gtaccgcgag gtcgtcgagt tcgcgaagaa catcggcatg    540

gtgccgatcg aactcaagaa ggagaaggcg ggctacgtac tcaactcgct cctggtcccg    600

ctcctcaacg cggcctccga cctgctgatc gacggcatcg ccgatcccga catggtcgac    660

aagacgtggc gtatcggcac cggagccccg tggggcccct tccagatcat ggacgtcgtc    720

gggttgacca ccgtctacaa catctcctcc cagggcggcg agaagcagcg cgaattcgcc    780

gactacatca agaagaacta catcgacgag ggcaagctcg gcgttgctgt cggcgacggc    840

ttctacaact acaagggctg a                                              861


<210>  11
<211>  861
<212>  DNA
<213>  人工序列

<220>
<223>  双羰基还原酶突变体编码序列


<220>
<221>  misc_feature
<222>  (282)..(282)
<223>  n=a、c、g、或t


<220>
<221>  misc_feature
<222>  (253)..(253)
<223>  r=g或a

<400>  11
atgactgaac tgaagcagat caccgttctg ggtaccggag ttctcggctc acagatcgcc     60

tatcagaccg cctgtcacgg tttcgacgtc gtcgcgtacg acatcaacgc cgaggtcatc    120

gaaaaggcca aggctcggtt cgactcgttg gccgcggcct acaaggccga gaacgtcgag    180

ggtgccaagg aaggcaaggc tgacgaagcg ctgcaacgta ttacgtactc gtacgatcta    240

ggcgaagccg tcgccaaggc cgacctggtc atcgaggcag tncccgagga catcgccatc    300

aagcgcgaca cctacgagaa gcttgccacg gttgctcctg agcacacggt gttcgctacc    360

aactcctcga cgctgctgcc gagcgatctc aaggagttca ccggccgtcc cgagaagttc    420

ctcgcactgc acttcgcaaa tcacgtgtgg caraacaaca ctgccgaggt catgggcacc    480

gagtccaccg accccgccgt gtaccgcgag gtcgtcgagt tcgcgaagaa catcggcatg    540

gtgccgatcg aactcaagaa ggagaaggcg ggctacgtac tcaactcgct cctggtcccg    600

ctcctcaacg cggcctccga cctgctgatc gacggcatcg ccgatcccga catggtcgac    660

aagacgtggc gtatcggcac cggagccccg tggggcccct tccagatcat ggacgtcgtc    720

gggttgacca ccgtctacaa catctcctcc cagggcggcg agaagcagcg cgaattcgcc    780

gactacatca agaagaacta catcgacgag ggcaagctcg gcgttgctgt cggcgacggc    840

ttctacaact acaagggctg a                                              861


<210>  12
<211>  861
<212>  DNA
<213>  人工序列

<220>
<223>  双羰基还原酶突变体编码序列


<220>
<221>  misc_feature
<222>  (717)..(717)
<223>  h=a或c或t/u


<220>
<221>  misc_feature
<222>  (771)..(771)
<223>  r=g或a

<400>  12
atgactgaac tgaagcagat caccgttctg ggtaccggag ttctcggctc acagatcgcc     60

tatcagaccg cctgtcacgg tttcgacgtc gtcgcgtacg acatcaacgc cgaggtcatc    120

gaaaaggcca aggctcggtt cgactcgttg gccgcggcct acaaggccga gaacgtcgag    180

ggtgccaagg aaggcaaggc tgacgaagcg ctgcaacgta ttacgtactc gtacgatcta    240

ggcgaagccg tcgccaaggc cgacctggtc atcgaggcaa ttcccgagga catcgccatc    300

aagcgcgaca cctacgagaa gcttgccacg gttgctcctg agcacacggt gttcgctacc    360

aactcctcga cgctgctgcc gagcgatctc aaggagttca ccggccgtcc cgagaagttc    420

ctcgcactgc acttcgcaaa tcacgtgtgg gtcaacaaca ctgccgaggt catgggcacc    480

gagtccaccg accccgccgt gtaccgcgag gtcgtcgagt tcgcgaagaa catcggcatg    540

gtgccgatcg aactcaagaa ggagaaggcg ggctacgtac tcaactcgct cctggtcccg    600

ctcctcaacg cggcctccga cctgctgatc gacggcatcg ccgatcccga catggtcgac    660

aagacgtggc gtatcggcac cggagccccg ttcggcccct tccagatcat ggacathgtc    720

gggttgacca ccgtctacaa catctcctcc cagggcggcg agaagcagaa rgaattcgcc    780

gactacatca agaagaacta catcgacgag ggcaagctcg gcgttgctgt cggcgacggc    840

ttctacaact acaagggctg a                                              861


<210>  13
<211>  861
<212>  DNA
<213>  人工序列

<220>
<223>  双羰基还原酶突变体编码序列


<220>
<221>  misc_feature
<222>  (453,771)
<223>  r=g或a

<400>  13
atgactgaac tgaagcagat caccgttctg ggtaccggag ttctcggctc acagatcgcc     60

tatcagaccg cctgtcacgg tttcgacgtc gtcgcgtacg acatcaacgc cgaggtcatc    120

gaaaaggcca aggctcggtt cgactcgttg gccgcggcct acaaggccga gaacgtcgag    180

ggtgccaagg aaggcaaggc tgacgaagcg ctgcaacgta ttacgtactc gtacgatcta    240

ggcgaagccg tcgccaaggc cgacctggtc atcgaggcaa ttcccgagga catcgccatc    300

aagcgcgaca cctacgagaa gcttgccacg gttgctcctg agcacacggt gttcgctacc    360

aactcctcga cgctgctgcc gagcgatctc aaggagttca ccggccgtcc cgagaagttc    420

ctcgcactgc acttcgcaaa tcacgtgtgg caraacaaca ctgccgaggt catgggcacc    480

gagtccaccg accccgccgt gtaccgcgag gtcgtcgagt tcgcgaagaa catcggcatg    540

gtgccgatcg aactcaagaa ggagaaggcg ggctacgtac tcaactcgct cctggtcccg    600

ctcctcaacg cggcctccga cctgctgatc gacggcatcg ccgatcccga catggtcgac    660

aagacgtggc gtatcggcac cggagccccg ttcggcccct tccagatcat ggacgtcgtc    720

gggttgacca ccgtctacaa catctcctcc cagggcggcg agaagcagaa rgaattcgcc    780

gactacatca agaagaacta catcgacgag ggcaagctcg gcgttgctgt cggcgacggc    840

ttctacaact acaagggctg a                                              861


<210>  14
<211>  861
<212>  DNA
<213>  人工序列

<220>
<223>  双羰基还原酶突变体编码序列


<220>
<221>  misc_feature
<222>  (282)..(282)
<223>  n=a、c、g、或t


<220>
<221>  misc_feature
<222>  (453)..(453)
<223>  r=g或a

<400>  14
atgactgaac tgaagcagat caccgttctg ggtaccggag ttctcggctc acagatcgcc     60

tatcagaccg cctgtcacgg tttcgacgtc gtcgcgtacg acatcaacgc cgaggtcatc    120

gaaaaggcca aggctcggtt cgactcgttg gccgcggcct acaaggccga gaacgtcgag    180

ggtgccaagg aaggcaaggc tgacgaagcg ctgcaacgta ttacgtactc gtacgatcta    240

ggcgaagccg tcgccaaggc cgacctggtc atcgaggcag tncccgagga catcgccatc    300

aagcgcgaca cctacgagaa gcttgccacg gttgctcctg agcacacggt gttcgctacc    360

aactcctcga cgctgctgcc gagcgatctc aaggagttca ccggccgtcc cgagaagttc    420

ctcgcactgc acttcgcaaa tcacgtgtgg caraacaaca ctgccgaggt catgggcacc    480

gagtccaccg accccgccgt gtaccgcgag gtcgtcgagt tcgcgaagaa catcggcatg    540

gtgccgatcg aactcaagaa ggagaaggcg ggctacgtac tcaactcgct cctggtcccg    600

ctcctcaacg cggcctccga cctgctgatc gacggcatcg ccgatcccga catggtcgac    660

aagacgtggc gtatcggcac cggagccccg ttcggcccct tccagatcat ggacgtcgtc    720

gggttgacca ccgtctacaa catctcctcc cagggcggcg agaagcagcg cgaattcgcc    780

gactacatca agaagaacta catcgacgag ggcaagctcg gcgttgctgt cggcgacggc    840

ttctacaact acaagggctg a                                              861


<210>  15
<211>  29
<212>  DNA
<213>  人工序列

<220>
<223>  引物I94-Forward


<220>
<221>  misc_feature
<222>  (14)..(16)
<223>  n=a、t， g或c

<400>  15
ggtcatcgag gcannncccg aggacatcg                                       29


<210>  16
<211>  29
<212>  DNA
<213>  人工序列

<220>
<223>  引物I94-Reverse


<220>
<221>  misc_feature
<222>  (14)..(16)
<223>  n=a、t、g或c

<400>  16
cgatgtcctc gggnnntgcc tcgatgacc                                       29


<210>  17
<211>  38
<212>  DNA
<213>  人工序列

<220>
<223>  引物E96-Forward


<220>
<221>  misc_feature
<222>  (19)..(21)
<223>  n=a、t、g或c

<400>  17
gtcatcgagg caattcccnn ngacatcgcc atcaagcg                             38


<210>  18
<211>  38
<212>  DNA
<213>  人工序列

<220>
<223>  引物E96-Reverse


<220>
<221>  misc_feature
<222>  (18)..(20)
<223>  n=a、t、g或c

<400>  18
cgcttgatgg cgatgtcnnn gggaattgcc tcgatgac                             38


<210>  19
<211>  40
<212>  DNA
<213>  人工序列

<220>
<223>  引物R102-Forward


<220>
<221>  misc_feature
<222>  (19)..(21)
<223>  n=a、t、g或c

<400>  19
gaggacatcg ccatcaagnn ngacacctac gagaagcttg                           40


<210>  20
<211>  40
<212>  DNA
<213>  人工序列

<220>
<223>  引物R102-Reverse


<220>
<221>  misc_feature
<222>  (20)..(22)
<223>  n=a、t、g或c

<400>  20
caagcttctc gtaggtgtcn nncttgatgg cgatgtcctc                           40


<210>  21
<211>  30
<212>  DNA
<213>  人工序列

<220>
<223>  引物T124-Forward


<220>
<221>  misc_feature
<222>  (15)..(17)
<223>  n=a、t、g或c

<400>  21
ctaccaactc ctcgnnnctg ctgccgagcg                                      30


<210>  22
<211>  30
<212>  DNA
<213>  人工序列

<220>
<223>  引物T124-Reverse


<220>
<221>  misc_feature
<222>  (14)..(16)
<223>  n=a、t、g或c

<400>  22
cgctcggcag cagnnncgag gagttggtag                                      30


<210>  23
<211>  30
<212>  DNA
<213>  人工序列

<220>
<223>  引物S123-Forward


<220>
<221>  misc_feature
<222>  (14)..(16)
<223>  n=a、t、g或c

<400>  23
cgctaccaac tccnnnacgc tgctgccgag                                      30


<210>  24
<211>  30
<212>  DNA
<213>  人工序列

<220>
<223>  引物S123-Reverse


<220>
<221>  misc_feature
<222>  (15)..(17)
<223>  n=a、t、g或c

<400>  24
ctcggcagca gcgtnnngga gttggtagcg                                      30


<210>  25
<211>  27
<212>  DNA
<213>  人工序列

<220>
<223>  引物H148-Forward


<220>
<221>  misc_feature
<222>  (13)..(15)
<223>  n=a、t、g或c

<400>  25
cacttcgcaa atnnngtgtg ggtcaac                                         27


<210>  26
<211>  28
<212>  DNA
<213>  人工序列

<220>
<223>  引物H148-Reverse


<220>
<221>  misc_feature
<222>  (13)..(15)
<223>  n=a、t、g或c

<400>  26
gttgacccac acnnnatttg cgaagtgg                                        28


<210>  27
<211>  29
<212>  DNA
<213>  人工序列

<220>
<223>  引物V151-Forward


<220>
<221>  misc_feature
<222>  (15)..(17)
<223>  n=a、t、g或c

<400>  27
caaatcacgt gtggnnnaac aacactgcc                                       29


<210>  28
<211>  29
<212>  DNA
<213>  人工序列

<220>
<223>  引物V151-Reverse


<220>
<221>  misc_feature
<222>  (13)..(15)
<223>  n=a、t、g或c

<400>  28
ggcagtgttg ttnnnccaca cgtgatttg                                       29


<210>  29
<211>  29
<212>  DNA
<213>  人工序列

<220>
<223>  引物E156-Forward


<220>
<221>  misc_feature
<222>  (14)..(16)
<223>  n=a、t、g或c

<400>  29
caacaacact gccnnngtca tgggcaccg                                       29


<210>  30
<211>  29
<212>  DNA
<213>  人工序列

<220>
<223>  引物E156-Reverse


<220>
<221>  misc_feature
<222>  (14)..(16)
<223>  n=a、t、g或c

<400>  30
cggtgcccat gacnnnggca gtgttgttg                                       29


<210>  31
<211>  33
<212>  DNA
<213>  人工序列

<220>
<223>  引物K189-Forward


<220>
<221>  misc_feature
<222>  (16)..(18)
<223>  n=a、t、g或c

<400>  31
gaactcaaga aggagnnngc gggctacgta ctc                                  33


<210>  32
<211>  33
<212>  DNA
<213>  人工序列

<220>
<223>  引物K189-Reverse


<220>
<221>  misc_feature
<222>  (16)..(18)
<223>  n=a、t、g或c

<400>  32
gagtacgtag cccgcnnnct ccttcttgag ttc                                  33


<210>  33
<211>  40
<212>  DNA
<213>  人工序列

<220>
<223>  引物G191-Forward


<220>
<221>  misc_feature
<222>  (22)..(24)
<223>  n=a、t、g或c

<400>  33
gaactcaaga aggagaaggc gnnntacgta ctcaactcgc                           40


<210>  34
<211>  40
<212>  DNA
<213>  人工序列

<220>
<223>  引物G191-Reverse


<220>
<221>  misc_feature
<222>  (17)..(19)
<223>  n=a、t、g或c

<400>  34
gcgagttgag tacgtannnc gccttctcct tcttgagttc                           40


<210>  35
<211>  27
<212>  DNA
<213>  人工序列

<220>
<223>  引物L194-Forward


<220>
<221>  misc_feature
<222>  (12)..(14)
<223>  n=a、t、g或c

<400>  35
cgggctacgt annnaactcg ctcctgg                                         27


<210>  36
<211>  27
<212>  DNA
<213>  人工序列

<220>
<223>  引物L194-Reverse


<220>
<221>  misc_feature
<222>  (14)..(16)
<223>  n=a、t、g或c

<400>  36
ccaggagcga gttnnntacg tagcccg                                         27


<210>  37
<211>  30
<212>  DNA
<213>  人工序列

<220>
<223>  引物W223-Forward


<220>
<221>  misc_feature
<222>  (14)..(16)
<223>  n=a、t、g或c

<400>  37
ggtcgacaag acgnnncgta tcggcaccgg                                      30


<210>  38
<211>  30
<212>  DNA
<213>  人工序列

<220>
<223>  引物W223-Reverse


<220>
<221>  misc_feature
<222>  (15)..(17)
<223>  n=a、t、g或c

<400>  38
ccggtgccga tacgnnncgt cttgtcgacc                                      30


<210>  39
<211>  40
<212>  DNA
<213>  人工序列

<220>
<223>  引物F231-Forward


<220>
<221>  misc_feature
<222>  (20)..(22)
<223>  n=a、t、g或c

<400>  39
tatcggcacc ggagccccgn nnggcccctt ccagatcatg                           40


<210>  40
<211>  40
<212>  DNA
<213>  人工序列

<220>
<223>  引物F231-Reverse


<220>
<221>  misc_feature
<222>  (19)..(21)
<223>  n=a、t、g或c

<400>  40
catgatctgg aaggggccnn ncggggctcc ggtgccgata                           40


<210>  41
<211>  32
<212>  DNA
<213>  人工序列

<220>
<223>  引物M237-Forward


<220>
<221>  misc_feature
<222>  (15)..(17)
<223>  n=a、t、g或c

<400>  41
gccccttcca gatcnnngac gtcgtcgggt tg                                   32


<210>  42
<211>  32
<212>  DNA
<213>  人工序列

<220>
<223>  引物M237-Reverse


<220>
<221>  misc_feature
<222>  (16)..(18)
<223>  n=a、t、g或c

<400>  42
caacccgacg acgtcnnnga tctggaaggg gc                                   32


<210>  43
<211>  33
<212>  DNA
<213>  人工序列

<220>
<223>  引物V239-Forward


<220>
<221>  misc_feature
<222>  (17)..(19)
<223>  n=a、t、g或c

<400>  43
cttccagatc atggacnnng tcgggttgac cac                                  33


<210>  44
<211>  33
<212>  DNA
<213>  人工序列

<220>
<223>  引物V239-Reverse


<220>
<221>  misc_feature
<222>  (15)..(17)
<223>  n=a、t、g或c

<400>  44
gtggtcaacc cgacnnngtc catgatctgg aag                                  33


<210>  45
<211>  27
<212>  DNA
<213>  人工序列

<220>
<223>  引物V240-Forward


<220>
<221>  misc_feature
<222>  (14)..(16)
<223>  n=a、t、g或c

<400>  45
gatcatggac gtcnnngggt tgaccac                                         27


<210>  46
<211>  27
<212>  DNA
<213>  人工序列

<220>
<223>  引物V240-Reverse


<220>
<221>  misc_feature
<222>  (12)..(14)
<223>  n=a、t、g或c

<400>  46
gtggtcaacc cnnngacgtc catgatc                                         27


<210>  47
<211>  29
<212>  DNA
<213>  人工序列

<220>
<223>  引物N247-Forward


<220>
<221>  misc_feature
<222>  (14)..(16)
<223>  n=a、t、g或c

<400>  47
gaccaccgtc tacnnnatct cctcccagg                                       29


<210>  48
<211>  29
<212>  DNA
<213>  人工序列

<220>
<223>  引物N247-Reverse


<220>
<221>  misc_feature
<222>  (14)..(16)
<223>  n=a、t、g或c

<400>  48
cctgggagga gatnnngtag acggtggtc                                       29


<210>  49
<211>  29
<212>  DNA
<213>  人工序列

<220>
<223>  引物Q251-Forward


<220>
<221>  misc_feature
<222>  (14)..(16)
<223>  n=a、t、g或c

<400>  49
caacatctcc tccnnnggcg gcgagaagc                                       29


<210>  50
<211>  29
<212>  DNA
<213>  人工序列

<220>
<223>  引物Q251-Reverse


<220>
<221>  misc_feature
<222>  (14)..(16)
<223>  n=a、t、g或c

<400>  50
gcttctcgcc gccnnnggag gagatgttg                                       29


<210>  51
<211>  44
<212>  DNA
<213>  人工序列

<220>
<223>  引物R257-Forward


<220>
<221>  misc_feature
<222>  (21)..(23)
<223>  n=a、t、g或c

<400>  51
cccagggcgg cgagaagcag nnngaattcg ccgactacat caag                      44


<210>  52
<211>  44
<212>  DNA
<213>  人工序列

<220>
<223>  引物R257-Reverse


<220>
<221>  misc_feature
<222>  (22)..(24)
<223>  n=a、t、g或c

<400>  52
cttgatgtag tcggcgaatt cnnnctgctt ctcgccgccc tggg                      44


<210>  53
<211>  31
<212>  DNA
<213>  人工序列

<220>
<223>  引物L273-Forward


<220>
<221>  misc_feature
<222>  (17)..(19)
<223>  n=a、t、g或c

<400>  53
catcgacgag ggcaagnnng gcgttgctgt c                                    31


<210>  54
<211>  31
<212>  DNA
<213>  人工序列

<220>
<223>  引物L273-Reverse


<220>
<221>  misc_feature
<222>  (13)..(15)
<223>  n=a、t、g或c

<400>  54
gacagcaacg ccnnncttgc cctcgtcgat g                                    31


<210>  55
<211>  40
<212>  DNA
<213>  人工序列

<220>
<223>  引物A276-Forward


<220>
<221>  misc_feature
<222>  (20)..(22)
<223>  n=a、t、g或c

<400>  55
cgagggcaag ctcggcgttn nngtcggcga cggcttctac                           40


<210>  56
<211>  40
<212>  DNA
<213>  人工序列

<220>
<223>  引物A276-Reverse


<220>
<221>  misc_feature
<222>  (19)..(21)
<223>  n=a、t、g或c

<400>  56
gtagaagccg tcgccgacnn naacgccgag cttgccctcg                           40



